| Engineered dermis loaded with confining forces promotes full-thickness wound healing by enhancing vascularisation and epithelialisation |
| 论文作者 |
Zhang, GL; Zhang, ZQ; Cao, GB; Jin, QH; Xu, L; Li, JY; Liu, Z; Xu, C; Le, YY; Fu, Y; Ju, JH; Li, B; Hou, RX |
| 期刊/会议名称 |
ACTA BIOMATERIALIA |
| 论文年度 |
2023 |
| 论文类别 |
Article |
| 摘要 |
Tissue-engineered skin is ideal for clinical wound repair. Restoration of skin tissue defects using tissue-engineered skin remains a challenge owing to insufficient vascularisation. In our previous study, we developed a 3D bioprinted model with confined force loading and demonstrated that the confined force can affect vascular branching, which is regulated by the YAP signalling pathway. The mechanical properties of the model must be optimised to suture the wound edges. In this study, we explored the ability of a GelMA-HAMA-fibrin scaffold to support the confined forces created by 3D bioprinting and promote vascularisation and wound healing. The shape of the GelMA-HAMA-fibrin scaffold containing 3% GelMA was affected by the confined forces produced by the embedded cells. The GelMA-HAMA-fibrin scaffold was easy to print, had optimal mechanical properties, and was biocompatible. The constructs were success-fully sutured together after 14 d of culture. Scaffolds seeded with cells were transplanted into skin tissue defects in nude mice, demonstrating that the cell-seeded GelMA-HAMA-fibrin scaffold, under confined force loading, promoted neovascularisation and wound restoration by enhancing blood vessel connec-tions, creating a patterned surface, growth factors, and collagen deposition. These results provide further insights into the production of hydrogel composite materials as tissue-engineered scaffolds under an internal mechanical load that can enhance vascularisation and offer new treatment methods for wound healing. |
| 卷 |
170 |
| 影响因子 |
9.7 |
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