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Isolation and Characterization of Extracellular Vesicles Secreted from Human Pluripotent Stem Cell-Derived Cardiovascular Progenitor Cells.
论文作者 Wu, Qiang; Ke, Min-Xia; Yang, Huang-Tian
期刊/会议名称 Methods in molecular biology (Clifton, N.J.)
论文年度 2022
论文类别
摘要 © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.Extracellular vesicles (EVs) secreted by human pluripotent stem cells-derived cardiovascular progenitor cells (hPSC-CVPCs) can improve repair of infarcted hearts in mouse and nonhuman primate myocardial infarction models. To fully achieve their values, it is essential to establish an efficient method for the isolation of EVs from hPSC-CVPCs. Here we describe the protocols for efficient isolation and characterization of EVs from the conditioned medium of hPSC-CVPCs.
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